Pfu POLYmerase
High quality enzymes for your molecular applications
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PolyPfu DNA Polymerase is a recombinant, thermostable Pfu DNA polymerase. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The PolyPfu DNA polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’→3’ direction, shows no 3’→ 5’ exonuclease activity, but has a 5’→ 3’ exonuclease activity.

Specs:

  • Recombinant thermostable DNA polymerases derived from Pyrococcus furiosus and overexpressed in E. coli.
  • Extreme yields with minimal amounts of enzyme and little optimization.
  • Increased sensitivity.
  • Suitable for a wide range of applications include cloning, gene expression, and site-directed mutagenesis
  • The half-life of the enzyme is 45 minutes at 95°C.
  • Amplifies fragments of up to 5 kb.
  • Leaves ´A´ overhangs.The enzyme is purified to a very high degree and is absent of nucleic acid

*SDS page (Coumassiee stained) shows the degree of purity of the enzyme